TrueORF® cDNA Clones
Ready-to-use tagged cDNA clones
TrueORF® cDNA clones enable the expression of the encoded transcript as C-terminally tagged protein with Myc and FLAG® epitopes. They facilitate multiple applications that utilize an anti-tag antibody, such as protein expression, protein purification and subcellular localization.
View all TrueORF® cDNA Clones
Features
- The CMV promoter and a Kozak consensus sequence drive protein expression in mammalian cells
- The C-terminus of the ORF is tagged with Myc/DDK or tGFP
- Provided as 10 µg transfection-ready plasmids
- Upstream T7 promoter allows protein expression in cell-free system
- Engineered as the entry vector for the Precision Shuttle system (restriction enzyme-based shuttling system)
Benefits
- 60 destination vectors for additional applications
- Easy shuttling
- Validated for Western Blot
- Two types of ready-to-use clones; Myc-DDK tagged or GFP tagged
- 20,000 human clones are validated for protein expression
| pCMV6-Entry | pCMV6-AC-GFP | pLenti-C-Myc-DDK | pLenti-C-mGFP | |
|---|---|---|---|---|
| Product Code | PS100001 | PS100010 | PS100064 | PS100071 |
| Fusion Tag | C-terminal Myc-DDK | C-terminal tGFP | C-terminal Myc-DDK | C-terminal mGFP |
| Vector Map |  |  |  |  |
| Application | Detection and purification of over-expressed protein | Tracking the over-expressed protein in transfected cells | Detection and purification of over-expressed protein in hard-to-transfect cells | Tracking the over-expressed protein in transfected cells |
| Download Datasheet |
TrueORF® Gold
TrueORF® Gold is collection of premium cDNA clones that have passed the ultimate tests: sequence verification and protein expression validation. Why settle for clones with little validation or wait for gene-synthesis? Get a TrueORF® Gold clone and start your experiment immediately.
Features
- Tested individually by Western blot
- Sequence verified
- Transfection ready
- Easily shuttled into over 60 vectors
