HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZFP36 (RC202049, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZFP36. Positive lysates LY401161 (100ug) and LC401161 (20ug) can be purchased separately from OriGene.
Anti-ZFP36 mouse monoclonal antibody (TA502379) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ZFP36 (RC202049).
HEK293T cells transfected with either RC202049 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ZFP36 antibody (TA502379), and then analyzed by flow cytometry.