Western blot analysis of TREF1 Antibody (C-term) (Cat. #TA325102) in K562 cell line lysates (35ug/lane). TREF1 (arrow) was detected using the purified Pab.
IF image of MCF-7 cell stained with TREF1 Antibody (C-term) (Cat#TA325102).MCF-7 cells were incubated with TREF1 primary antibody (1:25, 1 h at 37Â°C). For secondary antibody, Alexa FluorÂ® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa FluorÂ® 555 (red) conjugated Phalloidin (7 units/ml).TREF1 immunoreactivity is localized to Nucleus significantly.
TREF1 Antibody (C-term) (Cat. #TA325102) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.