HEK293T cells transfected with either RC202408 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SSR1 antibody (TA503265), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-SSR1 antibody (TA503265), (Red), compared to a nonspecific negative control antibody, (Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSR1 (RC202408, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSR1. Positive lysates LY401093 (100ug) and LC401093 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SSR1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and SSR1-Knockout HeLa cells (KO, Cat# LC812609) were separated by SDS-PAGE and immunoblotted with anti-SSR1 monoclonal antibody TA503265 (1:2000`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Flow cytometric Analysis of Jurkat cells, using anti-SSR1 antibody (TA503265), (Red), compared to a nonspecific negative control antibody, (Blue).