Western blot analysis of extracts (35ug) from 4 different cell lines by using anti-TSN monoclonal antibody (1:500).
Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and TSN-Knockout Hela cells (KO, Cat# LC810245) were separated by SDS-PAGE and immunoblotted with anti-TSN monoclonal antibody TA809193. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:200).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSN (RC201804, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSN (1:2000). Positive lysates LY417864 (100ug) and LC417864 (20ug) can be purchased separately from OriGene.