HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TKT (RC203497, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TKT. Positive lysates LY400434 (100ug) and LC400434 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TKT monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
Anti-TKT mouse monoclonal antibody (TA500892) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TKT (RC203497).
Flow cytometric analysis of Hela cells, using anti-TKT antibody (TA500892), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).
Flow cytometric analysis of Jurkat cells, using anti-TKT antibody (TA500892), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).