HEK293T cells transfected with either RC209184 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TF antibody (TA500848), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TF (RC209184, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TF (1:2000). Positive lysates LY400433 (100ug) and LC400433 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-TF monoclonal antibody (1:1000).
Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-TF monoclonal antibody (1:200).
Anti-TF mouse monoclonal antibody (TA500848) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TF (RC209184).