NF-kappaB Reporter Cellular Assay Pack (HEK293)

Product Code
AMS.79327
$0.00
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The NF-kappaB Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of the nuclear factor Kappa B (NF-kappaB) signal transduction pathways. The pack contains the NF-kappaB Reporter (Luc)-HEK293 Recombinant Cell Line/ a luciferase reporter cell line that contains a firefly luciferase gene under the control of four copies of the NF-kappaB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors/ endogenous NF-kappaB transcription factors bind to the DNA response elements/ inducing transcription of the luciferase reporter gene. This cell line is validated for the response to TNFalpha and to treatment with NF-kappaB inhibitor/ evodiamine.Additionally/ the pack includes cell culture medium (Thaw Medium 1) that has been optimized for use with HEK293 cells. Thaw Medium 1 includes 10% fetal bovine serum/ non-essential amino acids/ sodium pyruvate/ and 1% Pen/Strep. Finally/ the pack provides the ONE-Stepâ„¢ Luciferase Detection System. This reagent provides highly sensitive/ stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium/ and can be detected with any luminometer; automated injectors are not required.
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SKU AMS.79327
Size 2 vials
Species Human
Cell Line HEK293
Tissue Type Kidney
Application • Monitor NF-kappaB-signaling pathway activity • Screen inhibitors of NF-kappaB
Gene synonyms NF-kappaB Cell Line, NFkappaB Cell Line, Luciferase Reporter, NF-kB Cell Line, NFkB Cell Line
Storage Immediately upon receipt/ store vials in liquid nitrogen.
Shipping Temp -80C (dry ice)
Supplier Name BPS