NF-kappaB Reporter Cellular Assay Pack (HCT116)
Product Code
79326
$0.00
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The NF-kappaB Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of the nuclear factor Kappa B (NF-kappaB) signal transduction pathways. The pack contains the NF-kappaB Reporter (Luc)- HCT-116 Recombinant Cell Line/ a luciferase reporter cell line that contains a firefly luciferase gene under the control of four copies of the NF-kappaB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors/ endogenous NF-kappaB transcription factors bind to the DNA response elements/ inducing transcription of the luciferase reporter gene. This cell line is validated for the response to TNFalpha and to treatment with NF-kappaB inhibitor/ evodiamine.Additionally/ the pack includes cell culture medium (Thaw Medium 7) that has been optimized for use with HCT-116 cells. Thaw Medium 7 includes 10% fetal bovine serum and 1% Pen/Strep. Finally/ the pack provides the ONE-Stepâ„¢ Luciferase Detection System. This reagent provides highly sensitive/ stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium/ and can be detected with any luminometer; automated injectors are not required.
SKU | 79326 |
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Size | 2 vials |
Alternate SKU | AMS.79326 |
Species | Human |
Cell Line | HCT 116 |
Tissue Type | Colon |
Application | • Monitor NF-kappaB-signaling pathway activity • Screen inhibitors of NF-kappaB |
Gene synonyms | NF-kappaB Cell Line, NFkappaB Cell Line, Luciferase Reporter, NF-kB Cell Line, NFkB Cell Line |
Storage | Immediately upon receipt/ store vials in liquid nitrogen. |
Shipping Temp | -80C (dry ice) |
Supplier Name | BPS |