HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GTF2F1 (RC201294, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GTF2F1. Positive lysates LY400767 (100ug) and LC400767 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GTF2F1 monoclonal antibody.
Anti-GTF2F1 mouse monoclonal antibody (TA503380) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GTF2F1 (RC201294).
HEK293T cells transfected with either RC201294 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GTF2F1 antibody (TA503380), and then analyzed by flow cytometry.