Telomerase reverse transcriptase (TERT) Rabbit Polyclonal Antibody
Rabbit polyclonal TERT Antibody (Center)
WB, FC, IF
CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT
Western blot analysis of anti-TERT Antibody (Center) (RB14069) in Jurkat cell line lysates (35ug/lane). TERT (arrow) was detected using the purified Pab.
TERT Antibody (Center) (Cat. #TA324535) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IF image of SY5Y cells stained with TERT (Center) antibody. SY5Y cells were incubated with TA324535 TERT (Center) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor¶© 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue). TERT immunoreactivity is localized to the nucleus of SY5Y cells.
IF image of Hela cell stained with TERT Antibody (Center) (Cat#TA324535).Hela cells were incubated with TERT primary antibody (1:25, 1 h at 37¶øC). For secondary antibody, Alexa Fluor¶© 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor¶© 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue). TERT immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.