TCF/LEF Reporter Kit (Wnt Signaling Pathway)
Product Code
AMS.60500
$0.00
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The TCF/LEF Reporter kit is designed for monitoring the activity of Wnt / ?-catenin signaling pathway in the cultured cells. The kit contains transfection-ready TCF/LEF luciferase reporter vector|which is a Wnt pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized TCF/LEF responsive element located upstream of a minimal promoter. The TCF/LEF reporter is premixed with constitutively-expressing Renilla luciferase vector that serves as internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter|without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity.
SKU | AMS.60500 |
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Size | 500 reactions |
Application | • Monitor Wnt /?-catenin signaling pathway activity. • Screen for activators or inhibitors of the Wnt /?-catenin signaling pathway. • Study effects of RNAi or gene overexpression on the activity of the Wnt /?-catenin pathway. |
Gene synonyms | WNT reporter, TCF reporter, LEF reporter, luciferase reporter |
Format | assay kit |
Storage | Stable at least 12 months from date of receipt# when stored as directed (-20C) |
Shipping Temp | -20C |
Supplier Name | BPS |
Research Areas | Immunology |