Anti-WWTR1 mouse monoclonal antibody (TA503438) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY WWTR1 (RC204082).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WWTR1 (RC204082, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WWTR1.
HEK293T cells transfected with either RC204082 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-WWTR1 antibody (TA503438), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-WWTR1 monoclonal antibody.