TBP Antibody (Center) (Cat. #TA324859) western blot analysis in K562, U251 cell line lysates (35ug/lane).This demonstrates the TBP antibody detected the TBP protein (arrow).
TBP Antibody (Center) (Cat. #TA324859) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human testis tissue reacted with TBP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
IF image of U251 cell stained with TBP Antibody (Center) (Cat#TA324859).U251 cells were incubated with TBP primary antibody (1:25, 1 h at 37Â°C). For secondary antibody, Alexa FluorÂ® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa FluorÂ® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue).TBP immunoreactivity is localized to Nucleus significantly.