Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TACC3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and TACC3-Knockout 293T cells (KO, Cat# LC812337) were separated by SDS-PAGE and immunoblotted with anti-TACC3 monoclonal antibody TA501264, (1:200). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TACC3 (RC210754, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TACC3. Positive lysates LY416710 (100ug) and LC416710 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC210754 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TACC3 antibody (TA501264), and then analyzed by flow cytometry.
Anti-TACC3 mouse monoclonal antibody (TA501264) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TACC3 (RC210754).