Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TACC3 monoclonal antibody.
Anti-TACC3 mouse monoclonal antibody (TA501263) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TACC3 (RC210754).
HEK293T cells transfected with either RC210754 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TACC3 antibody (TA501263), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TACC3 (RC210754, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TACC3. Positive lysates LY416710 (100ug) and LC416710 (20ug) can be purchased separately from OriGene.
Flow cytometric Analysis of Jurkat cells, using anti-TACC3 antibody (TA501263), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).