Synthetic mRNA for iPS Cell Generation
Safely and efficiently reprogram somatic cells to induced pluripotent stem cells
The reprogramming of somatic cells to pluripotency was first described by Yamanaka, with this technique remaining the most established way to derive iPS cells. However, this method involves transduction of lentivirus or retrovirus encoding reprogramming factors, and results in viral integration into the genome. This viral integration combined with low reprogramming efficiencies presents some major barriers to the therapeutic use of iPS cells.
Synthetic highly modified mRNA overcomes these obstacles by inducing pluripotency without incurring genetic change, and removes any concerns associated with the use of viral vectors. In addition, synthetic mRNA is a powerful tool to direct stem cell fate and transdifferentiation.
Benefits
- Pre-made, highly purified and ready-to-use
- Simple, safe, non-integrating technique
- Modified to overcome innate antiviral responses
- Reprogram multiple human cell types to pluripotency
- Higher efficiencies than traditional protocols
- Direct cell fate and transdifferentiation
Protocol for iPSC Generation
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Citation
3D In Vitro Model of a Functional Epidermal Permeability Barrier from Human Embryonic Stem Cells and Induced Pluripotent Stem Cells
Ilic, D., et al. (2014) Stem Cell Reports 2, 1-15.
