IF image of A549 cell stained with SUMO1 Antibody (N-term) (Cat#TA324522).A549 cells were incubated with SUMO1 primary antibody (1:25, 1 h at 37Â°C). For secondary antibody, Alexa FluorÂ® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa FluorÂ® 555 (red) conjugated Phalloidin (7 units/ml).SUMO1 immunoreactivity is localized to Nuclear Membrane significantly.
Western blot analysis of lysate from mouse Neuro-2a cell line, using SUMO1 Antibody (N-term) (Cat. #TA324522). TA324522 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
SUMO1 Antibody (N-term) (Cat. #TA324522)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SUMO1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.