Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SULT2A1 monoclonal antibody.
HEK293T cells transfected with either RC204737 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SULT2A1 antibody (TA501658), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SULT2A1 (RC204737, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SULT2A1. Positive lysates LY418856 (100ug) and LC418856 (20ug) can be purchased separately from OriGene.