HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SULT1A1 (RC201601, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SULT1A1. Positive lysates LY420735 (100ug) and LC420735 (20ug) can be purchased separately from OriGene.
Anti-SULT1A1 mouse monoclonal antibody (TA501909) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SULT1A1 (RC201601 ).
HEK293T cells transfected with either RC201601 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SULT1A1 antibody (TA501909), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SULT1A1 monoclonal antibody.