Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-STK39 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunofluorescent staining of COS7 cells using anti-STK39 mouse monoclonal antibody (TA500496).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STK39 (RC223981, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STK39. Positive lysates LY402227 (100ug) and LC402227 (20ug) can be purchased separately from OriGene.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-STK39 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500496)
HEK293T cells transfected with either RC223981 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-STK39 antibody (TA500496), and then analyzed by flow cytometry.