iPSC-Derived Cells
Speed up your research without sacrificing cell purity
iPSC-derived cells provide a reliable and physiologically relevant alternative to primary cells and animal models, offering researchers a consistent and scalable source of human cells for drug discovery, disease modeling, and toxicity screening. Our ready-to-use iPSC-derived cardiomyocytes, hepatocytes, and neurons are fully differentiated, functionally validated, and available in high-purity formats to accelerate your research with minimal preparation time.
These are part of our iPSC Differentation suite. If you want to differentiate your own cells, check out Stem Cell Synergy - Differentiation for additional media and materials including iPSC Differentiation kits, or we can provide a stem cell differentation service for your cell lines depending on your time and research needs.
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Features and Benefits | Cardiosight®-S hiPSC-derived Cardiomyocytes | Hepatosight®-S hiPSC-derived Hepatocytes | Neurosight®-S hiPSC-derived Neurons
Features and Benefits
Fully Differentiated and Ready-to-Use
- Eliminates the need for in-house differentiation, saving time and resources
- Available as high-purity cardiomyocytes, hepatocytes, and neurons
- Functionally validated for key physiological properties
- Reproducible and High-Quality Cells
Batch-to-batch consistency ensures reliable experimental outcomes
- Consistently high quality, ISO9001 certified
- High viability, purity, and functionality for use in disease modeling and drug screening
- Scalability for Drug Discovery and Research
Highly Sensitive and functionally validated cells
- Electrophysiologically active cardiomyocytes, metabolically competent hepatocytes, and neurons for neurotoxicity testing
- Allow for easy detection of changes to electrophysiology by drug treatment
- Compatible with automated workflows for pharmaceutical research
- Serum-free media prevents unwanted interactions
Human-Relevant Alternative to Animal Models
- Provides more physiologically relevant data compared to traditional animal models
- Reduces ethical concerns by using human iPSC-derived cells
Cardiosight®-S hiPSC-derived Cardiomyocytes
The Cardiosight®-S is a highly pure and electrophysiologically active population of cells, suitable for all types of experiments in the field of cardiomyocytes. The cells have been validated using multiple platforms, including the Nanion Cardioexcyte96 and the Axion Maestro MEA, which showed synchronous beating with adequate FPD, higher calcium influx, and stronger contraction. The cells are designed to accelerate your workflow, resuming spontaneous beating just 2 days after thawing!
PRODUCT SPECIFICATION
- Cell Type: hiPSC-derived Cardiomyocytes
- Organism: Human
- Tissue Source: Fibroblast
- Purity: > 90% cardiomyocytes
- Shipped: Frozen
PRODUCT CHARACTERISTICS
- Human cardiac electrophysiology model
- High purity (>90% cTNT by flow cytometry)
- Stable, spontaneous, and synchronous beating
- Correct localization of Connexin43 (Cx43)
- Ratio of ventricular : atrial : nodal cells = ~80 :~19 : ~1
- High sensitivity to cardiotoxic drugs
The Cardiosight®-S recovers quickly upon thawing to start spontaneous beating the 2 days after and stabilizing over time (45~60 bpm on DIV7). The Cardiosight®-S cardiomyocytes readily form a single synchronous monolayer which enhances both reproducibility and accuracy of assays. The above images were taken from a 12-well culture plate 72 hours, 1 week and 2 weeks post-thawing (Magnification 100x).
Please note that the Cardiosight®-S was seeded following the Cardiosight®-S User Guide but at a plating density of 150,000 cells/㎠ to demonstrate correct layer formation even at lower densities. Specific applications such as MEA assays are optimized to different densities and will not display the same morphology as here.
The Cardiosight®-S expresses typical ventricular cardiomyocyte markers and structural characteristics which are indicative of appropriate contractile functionality of these cells. In particular, the Cardiosight®-S demonstrates high expression of Connexin 43, a gap junction protein, which suggests high interconnectivity and thus synchronicity of the myocytes a must for good MEA assay results. 30,000 cells were seeded on Matrigel on a 9 mm coverslip and cultured for 7 days before fixation. Immunocytochemistry was performed by incubating primary antibodies at the concentrations recommended by the respective suppliers at RT for 1 hour.
Electrophysiology validated on multiple platforms
The Cardiosight®-S proved a good fit with the Nanion Patchliner for recording patch clamp measurements of the sponataneous beating. Calcium channel activation by BayK and inhibition by Nifedipine could also be detected in changes of the APD90. These results demonstrate the Cardiosight®-S expresses the correct ion channels and can readily be used in pharmacology applications.
The Cardiosight®-S demonstrated low inter-well variability when tested on the Nanion CardioExcyte 96. High reproducibility of Impedance and EFP signals facilitated compound testing experiments (Nifedipine & Dofetilide). Concentrations were increased by consecutive treatments of 10% media changes and matched the expected results, validating the cardiomyocyte properties.
On Nanion’s all-new FLEXcyte 96 which uses soft subtrates to mature the cardiomyocytes, the Cardiosight®-S demonstrated high responsiveness to Isoproterenol and were able to detect positive inotropic effects after just 7 days of culture.
The Cardiosight®-S demonstrated low variability, good synchronicity and good compound reactivity(Nifedipine, E4031, Dofetilide, Mexiletine) on the Axion Maestro.
Recommended Applications
| Product Code | Product Name | Kit Contents | Applications |
|---|---|---|---|
| AMS.C-001 | Cardiosight®-S Media Kit | 1. Cardiosight®-S Media (100 ml), 2. Cardiosight®-S Maintenance Supplement (2 ml, 100X), 3. Cardiosight®-S Plating Supplement (0.6ml, 50X) | long-term cell culture |
| AMS.C-002 | Cardiosight®-S Media Kit | 1. Cardiosight®-S Media (200 ml), 2. Cardiosight®-S Maintenance Supplement (2 ml X 2, 100X), 3. Cardiosight®-S Plating Supplement (0.9ml, 50X) | long-term cell culture |
| AMS.CMS-001 | Advanced Medium Kit for Cardiosight®-S | 1. Cardiosight®-S Advanced Plating Media (30 ml), 2. Cardiosight®-S Advanced Maintenance Media (100 ml), 3. Cardiosight® -S Advanced Plating Supplement (0.6 ml, 50X), 4. Cardiosight®-S Advanced Maintenance Supplement 1 (1 ml, 100X) | electrophysiological experiments |
| AMS.CMS-002 | Advanced Medium Kit for Cardiosight®-S | 1. Cardiosight®-S Advanced Plating Media (45 ml), 2. Cardiosight®-S Advanced Maintenance Media (200 ml), 3. Cardiosight® -S Advanced Plating Supplement (0.9 ml, 50X), 4. Cardiosight®-S Advanced Maintenance Supplement 1 (2 ml, 100X) | electrophysiological experiments |
Hepatosight®-S hiPSC-derived Hepatocytes
The Hepatosight®-S is a highly pure and functionally active population of cells, suitable for all types of experiments in the field of hepatocytes. The cells are reliable and recover quickly upon thawing. The hepatocytes are ideal for tissue-specific research, toxicity screening, and drug discovery by allowing measurement of various metabolic activities, including albumin secretion, glycogen production, or CYP activity.
PRODUCT SPECIFICATION
- Cell Type: hiPSC-derived Hepatocytes
- Organism: Human
- Tissue Source: Fibroblast
- Purity: > 95% Hepatocytes
- Shipped: Frozen
PRODUCT CHARACTERISTICS
- Human liver functional model
- Accurate human toxicity
- Long-term 3D organoid culture
The Hepatosight®-S recovers quickly upon thawing to quickly form a monolayer of polygonal shaped cells. The above images were taken from a 12-well culture plate 1 day, 3 days, 5 days, 8 days and 10 days post-thawing (Magnification 100x). The Hepatosight®-S was seeded following the Hepatosight®-S user guide.
The Hepatosight®-S expresses typical hepatocyte markers and structural characteristics which are indicative of appropriate functionality of these cells. 1,000,000 cells seeded on 12well plate and cultured for 10days before fixation. Immunocytochemistry was performed by incubating primary antibodies at the concentrations recommended by the respective suppliers at RT for 1 hour.
The Hepatosight®-S demonstrated high fidelity to primary hepatocytes in a hepatotoxicity screen. Cells were plated according to the Hepatosight®-S User Guide and tested on DIV 10.
Neurosight®-S hiPSC-derived Neurons
Neurosight®-S are high quality neurons that accurately model human neuronal activity in vitro. The cells form mature neuronal networks comprised of functional neurons appropriate for a variety of applications related to the electrophysiological behavior such as calcium fluxes or MEA assays and neurite outgrowth or degeneration. This makes them a perfect choice for tissue-specific research, toxicity screening, efficacy testing, and drug discovery.
PRODUCT SPECIFICATION
- Cell Type: hiPSC-derived Neurons
- Organism: Human
- Tissue Source: Fibroblast
- Purity: > 95% Neurons
- Shipped: Frozen
PRODUCT CHARACTERISTICS
- Human neuronal electrophysiology model
- Fast neuronal network formation
- Long-term maturation and synchrony
Pure Population of Human Neurons The Neurosight®-S recovers quickly upon thawing to quickly form an intertwined network of neurons.The above images were taken from a 12-well culture plate 2 day, 7 days, 12 days and 14 days post-thawing(Magnification 200x). The Neurosight®-S was seeded following the Neurosight®-S user guide.
The Neurosight®-S expresses typical neuronal markers and structural characteristics which are indicative of appropriate functionality of these cells. 30,000 cells were seeded on Matrigel on a 9 mm coverslip and cultured for 7 days before fixation. Immunocytochemistry was performed by incubating primary antibodies at the concentrations recommended by the respective suppliers at RT for 1 hour.
The Neurosight®-S demonstrated high capacity to form electrically active, highly mature neural networks. The activity increases with time as the cells and network mature with time.
Electrophysiological Response to Picrotoxin, a seizurogenic drug (HESI NeuTox Seizure Liability Program). Network synchrony increase upon treatment of picrotoxin.
Recommended Applications
| Product Code | Product Name | Kit Contents | Applications |
|---|---|---|---|
| AMS.NMS-001 | Medium Kit for Neurosight®-S | 1. Neurosight®-S Media (NM-001, 50 ml), 2. Neurosight®-S Media Supplement (NS-001, 0.5 ml, 100X) | for cell culture |
| AMS.NMS-002 | Medium Kit for Neurosight®-S | 1. Neurosight®-S Media (NM-002, 100 ml), 2. Neurosight®-S Media Supplement (NS-002, 1 ml, 100X) | for cell culture |
| AMS.NMS-003 | Electrophysiology Media Kit for Neurosight®-S | 1. Neurosight®-S EP Media (50 ml), 2. Neurosight®-S Plating Media (20ml), 3. Neurosight®-S EP Media Supplement (1 ml, 50X), 4. Neurosight®-S Maintenance Supplement (0.15 ml, 500X) | recommended for MEA plates |
| AMS.NMS-004 | Electrophysiology Media Kit for Neurosight®-S | 1. Neurosight®-S EP Media (100 ml), 2. Neurosight®-S Plating Media (20ml), 3. Neurosight®-S EP Media Supplement (2 ml, 50X), 4. Neurosight®-S Maintenance Supplement (0.25 ml, 500X) | recommended for MEA plates |
Stem Cell Differentiation Services
We can differentiate your human pluripotent stem cells into a range of cell types for you! Simply send us your cells and receive differentiating or terminally differentiated cells in just 1-2 weeks.
