Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-STAT5A monoclonal antibody (1:200).
Anti-STAT5A mouse monoclonal antibody (TA502737) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY STAT5A (RC205753).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT5A (RC205753, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT5A. Positive lysates LY418866 (100ug) and LC418866 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC205753 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-STAT5A antibody (TA502737), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-STAT5A antibody (TA502737), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).