Anti-STAT4 mouse monoclonal antibody (TA503024) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY STAT4 (RC206892).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT4 (RC206892, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT4. Positive lysates LY401095 (100ug) and LC401095 (20ug) can be purchased separately from OriGene.
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-STAT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA503024)
HEK293T cells transfected with either RC206892 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-STAT4 antibody (TA503024), and then analyzed by flow cytometry.