Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-STAT1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:1000).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT1 (RC201075, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT1 (1:2000).
Equivalent amounts of cell lysates (10 ug per lane) of wild-type A549 cells and STAT1-Knockout A549 cells (KO, Cat# LC806225) were separated by SDS-PAGE and immunoblotted with anti-STAT1 monoclonal antibody TA500521 (1:500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.