Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-STAT1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:1000).
Western blot analysis of extracts (10ug) from 7 Human tissue by using anti-STAT1 monoclonal antibody ( 1: Uterus; 2: Breast; 3: Brain; 4: Liver; 5: Ovary; 6: Thyroid gland; 7: colon) at 1:200 dilution.
Immunofluorescent staining of HT29 cells using anti-STAT1 mouse monoclonal antibody (TA500542) (1:50).
Anti-STAT1 mouse monoclonal antibody (TA500542) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY STAT1 (RC201075) (1:100).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT1 (RC213858, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT1 (1:2000).
Equivalent amounts of cell lysates (10 ug per lane) of wild-type A549 cells and STAT1-Knockout A549 cells (KO, Cat# LC806225) were separated by SDS-PAGE and immunoblotted with anti-STAT1 monoclonal antibody TA500542 (1:500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.