Immunofluorescent staining of HepG2 cells using anti-SSB mouse monoclonal antibody (TA500406).
Figure from citation: Immunoprecipitation of SSB (La) protein level by using anti-SSB antibody in HeLa cells. View Citation
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSB (RC205013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSB. Positive lysates LY401091 (100ug) and LC401091 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-SSB monoclonal antibody (1:200).
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-SSB monoclonal antibody at 1:1000 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Anti-SSB mouse monoclonal antibody (TA500406) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SSB (RC205013).
Immunoprecipitation (IP) of SSB by using TrueMab monoclonal anti-SSB antibodies (Negative control: IP without adding anti-SSB antibody.). For each experiment, 500ul of DDK tagged SSB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SSB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Flow cytometric Analysis of Jurkat cells, using anti-SSB antibody (TA500406), (Red), compared to a nonspecific negative control antibody, (Blue).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SSB monoclonal antibody.