SRE Reporter Kit (MAPK/ERK Signaling Pathway )
Product Code
AMS.60511
$0.00
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The SRE Reporter Kit is designed for monitoring the activity of the ERK signaling pathway and the transcriptional activity of SRF in cultured cells. The kit contains a transfection-ready SRE luciferase reporter vector|which is an ERK pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized SRE responsive elements located upstream of a minimal promoter. The SRE reporter is premixed with a constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter|without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity.
SKU | AMS.60511 |
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Size | 500 reactions |
Application | • Monitor MAPK/ERK signaling pathway activity and SRF-mediated activity. • Screen for activators or inhibitors of the MAPK/ERK signaling pathway. • Study effects of RNAi or gene overexpression on the activity of the MAPK/ERK pathway. |
Gene synonyms | SRE reporter, MAPK reporter, ERK reporter |
Format | assay kit |
Storage | Stable at least 12 months from date of receipt# when stored as directed (-20C) |
Shipping Temp | -20C |
Supplier Name | BPS |
Research Areas | Immunology |