Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SQSTM1 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (RC203214, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1. Positive lysates LY401285 (100ug) and LC401285 (20ug) can be purchased separately from OriGene.
Anti-SQSTM1 mouse monoclonal antibody (TA502133) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SQSTM1 (RC203214).
HEK293T cells transfected with either RC203214 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SQSTM1 antibody (TA502133), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-SQSTM1 antibody (TA502133), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).