Anti-SPR mouse monoclonal antibody (TA502029) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SPR (RC205679).
HEK293T cells transfected with either RC205679 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SPR antibody (TA502029), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPR (RC205679, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPR. Positive lysates LY401086 (100ug) and LC401086 (20ug) can be purchased separately from OriGene.
Flow cytometric Analysis of Hela cells, using anti-SPR antibody (TA502029), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).