HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPR (RC205679, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPR. Positive lysates LY401086 (100ug) and LC401086 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SPR monoclonal antibody.
Anti-SPR mouse monoclonal antibody (TA501960) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SPR (RC205679).
Flow cytometric Analysis of Hela cells, using anti-SPR antibody (TA501960), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and SPR-Knockout Hela cells (KO, Cat# LC810223) were separated by SDS-PAGE and immunoblotted with anti-SPR monoclonal antibody TA501960. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:500).
HEK293T cells transfected with either RC205679 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SPR antibody (TA501960), and then analyzed by flow cytometry.