Spike (D614G) (SARS-CoV-2) Pseudotyped Lentivirus (Luc Reporter)
Product Code
AMS.78028-1
$0.00
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The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). As the first step of the viral replication/ the virus attaches to the host cell surface before entering the cell. The viral Spike protein recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes/ endothelial cells/ and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein and ACE2 may offer protection against the viral infection. A SARS-CoV-2 variant carrying the spike protein amino acid change D614G has become the most prevalent form in the global pandemic.
The SARS-CoV-2 Spike D614G Pseudotyped Lentivirus were produced with SARS-CoV-2 Spike (Genbank Accession #QHD43416.1; with D614G mutation) as the envelope glycoproteins instead of the commonly used VSV-G. These pseudovirions contain the firefly luciferase gene driven by a CMV promoter/ therefore/ the spike-mediated cell entry can be measured via luciferase activity. The SARS-CoV-2 Spike D614G pseudotyped lentivirus can be used to measure the activity of neutralizing antibody against SARS-CoV-2 in a Biosafety Level 2 facility.
The SARS-CoV-2 Spike D614G Pseudotyped Lentivirus were produced with SARS-CoV-2 Spike (Genbank Accession #QHD43416.1; with D614G mutation) as the envelope glycoproteins instead of the commonly used VSV-G. These pseudovirions contain the firefly luciferase gene driven by a CMV promoter/ therefore/ the spike-mediated cell entry can be measured via luciferase activity. The SARS-CoV-2 Spike D614G pseudotyped lentivirus can be used to measure the activity of neutralizing antibody against SARS-CoV-2 in a Biosafety Level 2 facility.
SKU | AMS.78028-1 |
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Size | 100 ul |
Species | SARS-CoV-2 |
Application | 1. Study the mechanism of viral transduction. 2. Screening for neutralizing antibodies for SARS-CoV-2 Spike and ACE2. |
Gene synonyms | covid, covid-19, covid19, coronavirus, Severe acute respiratory Coronavirus Spike 2, pseudovirus, pseudovirion, lentivirus reporter, neutralizing, ACE2, SARS-CoV-2 Spike D614G reporter, 78028-1, 78028-2 |
Storage | Lentiviruses are shipped with dry ice. For long term storage/ it is recommended to store the virus at -80C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle. |
Shipping Temp | -80C (dry ice) |
Supplier Name | BPS |