Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-SMS monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
HEK293T cells transfected with either RC200619 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SMS antibody (TA503097), and then analyzed by flow cytometry.
Anti-SMS mouse monoclonal antibody (TA503097) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SMS (RC200619).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMS (RC200619, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMS. Positive lysates LY417877 (100ug) and LC417877 (20ug) can be purchased separately from OriGene.
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SMS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA503097)
Flow cytometric Analysis of Hela cells, using anti-SMS antibody (TA503097), (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometric Analysis of Jurkat cells, using anti-SMS antibody (TA503097), (Red), compared to a nonspecific negative control antibody, (Blue).