Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 Antibody (Cat.#TA302025), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of SOX2 Antibody (Cat.#TA302025) by SOX2 recombinant protein. SOX2 (arrow) was detected using the purified Mab.
Western blot analysis of SOX2 (arrow) using mouse monoclonal SOX2 antibody (Cat.#TA302025). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies)
Fluorescent confocal image of SY5Y cells stained with SOX2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with TA302025 SOX2 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa FluorÃ‚Â® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Note the highly specific localization of the SOX2 mainly to the nucleus.
IF image of A549 cell stained with SOX2 Antibody (Cat#TA302025/SG110310AA).A549 cells were incubated with SOX2 primary antibody (1:25, 1 h at 37Â°C). For secondary antibody, Alexa FluorÂ® 488 conjugated donkey anti-mouse antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa FluorÂ® 555 (red) conjugated Phalloidin (7 units/ml).SOX2 immunoreactivity is localized to Nucleus significantly.