SoluBL21™ Competent E.coli Cells
Express insoluble proteins in soluble form
SoluBL21™ Competent E. coli is a significantly improved BL21 host strain for soluble mammalian protein expression. Using a directed evolution approach, a mutant strain of BL21(DE3) E. coli has been developed that can produce soluble protein in the majority of cases where expression in the parent BL21(DE3) yielded no detectable soluble product. With the SoluBL21™ strain, a major obstacle to effective protein expression in E. coli has been overcome for many mammalian proteins. This significant improvement should enable you to make progress in a wide range of applications more quickly and far less expensively than in the past.
Features
- Optimized E. coli strain for expressing proteins in soluble form
- Simple and straightforward protocol
- Compatible with all T7 promoter-based expression vectors
Benefits
- Improves the solubility profile of many recombinant proteins
- Permits the establishment of many clones that are toxic to, and cannot be established in, BL21(DE3) and BL21(DE3)pLysS
| Name | Datasheet | Packsize | Order |
|---|---|---|---|
| SoluBL21™ Chemically Competent E. coli |  | 10 x 50 ul | View |
Expression of insoluble proteins in SoluBL21™ vs. standard BL21(DE3) E. coli
Citations
Purification and characterization of the human γ-secretase activating protein
Catherine L. Deatherage, Arina Hadziselimovic, and Charles R. Sanders (2012) Biochemistry. 51 (25), 5153-5159
