Anti-SOCS3 mouse monoclonal antibody (TA503057) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SOCS3 (RC209305).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SOCS3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOCS3 (RC209305, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOCS3.
HEK293T cells transfected with either RC209305 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SOCS3 antibody (TA503057), and then analyzed by flow cytometry.