HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAP25 (RC202068, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAP25. Positive lysates LY418912 (100ug) and LC418912 (20ug) can be purchased separately from OriGene.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SNAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA502967)
Flow cytometric Analysis of Jurkat cells, using anti-SNAP25 antibody (TA502967), (Red), compared to a nonspecific negative control antibody, (Blue).
Anti-SNAP25 mouse monoclonal antibody (TA502967) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SNAP25 (RC202068).
HEK293T cells transfected with either RC202068 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SNAP25 antibody (TA502967), and then analyzed by flow cytometry.