Skeletal Muscle
Source, grow and differentiate
Skeletal muscle is one of the most important tissues in the human body, accounting for approximately 30-40% of the weight in a healthy individual. It plays a vital role in insulin-stimulated glucose disposal and often the site of insulin resistance in obesity. Human primary cultured skeletal myoblasts can directly reflect a patient's metabolic phenotype, because many of the signaling pathways are maintained intact. Therefore, modeling human skeletal muscle in vitro is important for understanding muscle function and for preclinical investigations into muscle-related diseases and drug discovery.
We have everything you need from sourcing starting material, differentiating and maturing skeletal muscle models in vitro. Read below to find out more about AMSBIO's Skeletal Muscle workflow.
AMSBIO's workflow
At AMSBIO we can streamline your modeling process by offering a range of products to support skeletal muscle cell culture. We offer human primary skeletal myoblasts from a variety of donors, including obese donors with Type 2 diabetes. To compliment our range of cell lines, we have formulated media available for maintenance, differentiation and maturation of skeletal muscle cells. Alternatively we offer our differentiation kits to direct iPSCs to form mature functional skeletal muscle cells.
Skeletal Muscle Cell Lines
Human skeletal myoblasts satellite cells are isolated from consented patients with good representation from all levels of adiposity and age. Skeletal myoblasts are available from both male and female donors. Pooled lots of cells are available to provide large lots for screening and to decrease issues with patient to patient variability.
We also offer a range of skeletal muscle and iPSC cell lines cryopreserved and ready-to-use. Visit our full biorepository range here.
| Name | Datasheet | Packsize | Order |
|---|---|---|---|
| Cryopreserved|Human Skeletal Muscle (500|000 cells/vial) Lean Donor|BMI<25.0 | - | each | View |
| Cryopreserved|Human Skeletal Myoblast (500|000 cells/vial)-DIABETIC DONOR | - | each | View |
| Cryopreserved|Human Skeletal Myoblasts (500|000 cells/vial) |  | each | View |
| Cryopreserved|Human Skeletal Myoblasts (500|000 cells/vial)|POOLED Donor lot | | each | View |
| Myoblasts (>= 3E+06/vial) | - | 1 | View |
Induction and Maturation Media
For Skeletal Muscle culture we offer differentiation and maturation media for different stages of skeletal muscle growth.
To differentiate iPSCs towards myoblasts we offer our induction medium. For myoblast maintenance we offer our myoblast cell growth media. For maturation we offer both myotube or myotube fusion medium to reach functionality. Lastly, for long term maintenance of mature skeletal muscle cells we have our skeletal muscle cell growth medium.
| Name | Datasheet | Packsize | Order |
|---|---|---|---|
| Skeletal Muscle Cell Growth Medium| 500ml | | 500ml | View |
| Skeletal Muscle. Induction Medium, 250mL Bottle | | 250ml | View |
| Skeletal Muscle. Myoblast Medium, 250mL Bottle | | 250ml | View |
| Skeletal Muscle. Myotube Fusion Medium, 250mL Bottle | | 250ml | View |
| Skeletal Muscle. Myotube Medium, 250mL Bottle | | 250ml | View |
Skeletal Muscle Differentiation
Skeletal Muscle Differentiation Kit
Differentiate human pluripotent stem cells using a simple 3-step process of media changes and cell passaging. The kit achieves typically 70% MF20-positive myotubes without the need for cell sorting or transfection. Alternatively, you can use this kit to differentiate human skeletal myoblasts to mature myotubes.
The process yields almost pure populations of myogenic cells; it is highly efficient and works robustly with all pluripotent stem cell lines – critical prerequisites for effective cell-based disease modeling and drug development.
As an alternative, we have a reprogramming kit Quick-Muscle™ Skeletal - SeV Kit to differentiate your human iPS/ES cells into skeletal muscle cells in just 7 days. Produces highly pure populations of skeletal muscle cells using a temperature-sensitive Sendai virus.
| Name | Datasheet | Packsize | Order |
|---|---|---|---|
| Quick-Muscle™ Skeletal - Maintenance Medium | - | 4 wells of a 24-well plate for 2 weeks | View |
| Quick-Muscle™ Skeletal - SeV Kit (Small) | - | 4 wells of a 24-well plate (Small) | View |
| Skeletal Muscle. Differentiation Media Kit, 4x250mL (+ 1 x Myoblast Vial)* | | 4 x 250 ml + 1 vial | View |
| Skeletal Muscle. Differentiation Media Kit, 4x250mL | | 4x250mL | View |
Analysis
Antibodies
See our full antibody range here.
Assays and Kits
See our full range of Assays and kits here.
