Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SIRT5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Flow cytometric Analysis of Hela cells, using anti-SIRT5 antibody (TA503279), (Red), compared to a nonspecific negative control antibody, (Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT5 (RC200189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT5. Positive lysates LY415885 (100ug) and LC415885 (20ug) can be purchased separately from OriGene.
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and SIRT5-Knockout HeLa cells (KO, Cat# LC830483) were separated by SDS-PAGE and immunoblotted with anti-SIRT5 monoclonal antibody TA503279 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
HEK293T cells transfected with either RC200189 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SIRT5 antibody (TA503279), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-SIRT5 antibody (TA503279), (Red), compared to a nonspecific negative control antibody, (Blue).