SIRT2 (Sirtuin2) Fluorogenic Assay Kit

Name: SIRT2 (Sirtuin2) Fluorogenic Assay Kit
Brand: AMS.50087
SKU: AMS.50087
Availability: InStock

Product Code

AMS.50087

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Overview

The Fluorogenic SIRT2 Assay Kit is a complete assay system designed to measure Sirtuin 2 (SIRT2) activity for screening and profiling applications. It comes in a convenient 96-well format|with all the reagents necessary for 32 fluorescent SIRT2 activity measurements*.The kit includes purified SIRT2 enzyme and a SIRT inhibitor|Nicotinamide|for use as a positive and negative control|respectively. The Fluorogenic SIRT2 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assaymethod eliminates dealing with the radioactivity|extraction|and chromatography aspects of traditional assays. Using this kit|only two simple steps on a microtiterplate are needed to analyze the SIRT2 activity level. First|the SIRT fluorometric substrate (SIRT substrate 1)|containing an acetylated lysine side chain|isincubated with purified SIRT2 enzyme. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that canthen be measured using a fluorescence reader.

Product Specifications

More Information
SKU	AMS.50087
Size	32 reactions
Application	Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Gene synonyms	sirt2, sirtuin 2
Storage	12 months from date of receipt# when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Shipping Temp	-80C (dry ice)
Supplier Name	BPS

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SIRT2 (Sirtuin2) Fluorogenic Assay Kit

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