SIRT2 (Sirtuin2) Fluorogenic Assay Kit
Product Code
AMS.50087
$0.00
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The Fluorogenic SIRT2 Assay Kit is a complete assay system designed to measure Sirtuin 2 (SIRT2) activity for screening and profiling applications. It comes in a convenient 96-well format|with all the reagents necessary for 32 fluorescent SIRT2 activity measurements*.The kit includes purified SIRT2 enzyme and a SIRT inhibitor|Nicotinamide|for use as a positive and negative control|respectively. The Fluorogenic SIRT2 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assaymethod eliminates dealing with the radioactivity|extraction|and chromatography aspects of traditional assays. Using this kit|only two simple steps on a microtiterplate are needed to analyze the SIRT2 activity level. First|the SIRT fluorometric substrate (SIRT substrate 1)|containing an acetylated lysine side chain|isincubated with purified SIRT2 enzyme. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that canthen be measured using a fluorescence reader.
SKU | AMS.50087 |
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Size | 32 reactions |
Application | Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. |
Gene synonyms | sirt2, sirtuin 2 |
Storage | 12 months from date of receipt# when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. |
Shipping Temp | -80C (dry ice) |
Supplier Name | BPS |