SIRT2 (Sirtuin2) Fluorogenic Assay Kit

Name: SIRT2 (Sirtuin2) Fluorogenic Assay Kit
Brand: AMS.50087
SKU: AMS.50087
Availability: InStock

Product Code

AMS.50087

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Overview

The Fluorogenic SIRT2 Assay Kit is a complete assay system_x000D_ designed to measure Sirtuin 2 (SIRT2) activity for screening and profiling_x000D_ applications. It comes in a convenient 96-well format, with all the reagents_x000D_ necessary for 32 fluorescent SIRT2 activity measurements*.The kit_x000D_ includes purified SIRT2 enzyme and a SIRT inhibitor, Nicotinamide, for use as a_x000D_ positive and negative control, respectively. The Fluorogenic SIRT2 Assay Kit is_x000D_ based on a unique fluorogenic substrate and developer combination. This assay_x000D_,method eliminates dealing with the radioactivity, extraction, and chromatography_x000D_ aspects of traditional assays. Using this kit, only two simple steps on a microtiter_x000D_,plate are needed to analyze the SIRT2 activity level. First, the SIRT fluorometric_x000D_ substrate (SIRT substrate 1), containing an acetylated lysine side chain, is_x000D_,incubated with purified SIRT2 enzyme. The deacetylation sensitizes the substrate so_x000D_ subsequent treatment with the Lysine Developer produces a fluorophore that can_x000D_,then be measured using a fluorescence reader._x000D_,

Product Specifications

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SKU	AMS.50087
Size	32 rxns.
Application	Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Gene synonyms	sirt2, sirtuin 2
Storage	12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Shipping Temp	-80°C (dry ice)
Supplier Name	BPS Bioscience Inc

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SIRT2 (Sirtuin2) Fluorogenic Assay Kit

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