SIRT1 (Sirtuin1) Fluorogenic Assay Kit

Name: SIRT1 (Sirtuin1) Fluorogenic Assay Kit
Brand: AMS.50081
SKU: AMS.50081
Availability: InStock

Product Code

AMS.50081

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Overview

The Fluorogenic SIRT1 Assay Kit is a complete assay system_x000D_ designed to measure Sirtuin 1 (SIRT1) activity for screening and profilingapplications. The Fluorogenic SIRT1_x000D_ Assay Kit is based on a unique fluorogenic substrate and developer combination._x000D_ This assay method eliminates dealing with the radioactivity, extraction, and_x000D_chromatography aspects of traditional assays. Using this kit, only two simple_x000D_ steps on a microtiter plate are needed to analyze the SIRT1 activity level. First,_x000D_ the SIRT fluorometric substrate (SIRT substrate 1), containing an acetylated_x000D_ lysine side chain, is incubated with purified SIRT1 enzyme. The deacetylation_x000D_ sensitizes the substrate so subsequent treatment with the Lysine Developer_x000D_ produces a fluorophore that can then be measured using a fluorescence reader.

Product Specifications

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SKU	AMS.50081
Size	96 rxns.
Application	Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Gene synonyms	sirt1, Sir2, Sir2a
Storage	12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Shipping Temp	-80°C (dry ice)
Supplier Name	BPS Bioscience Inc

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SIRT1 (Sirtuin1) Fluorogenic Assay Kit

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