SiMPLe Protein Labeling Kit (Sortase Mediated Protein Ligation)
Product Code
AMS.79392
$0.00
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Sortases catalize a transpeptidation reaction|where the enzyme cleaves the amide bond between the threonine and glycine of the sortase recognition sequence (LPXTG for S. aureus Sortase A)|generating a thioacyl intermediate. Subsequently|this intermediate is resolved by the N-terminus of an oligoglycine nucleophile|creating a new peptide bond that links the substrate to the incoming nucleophile. The Sortagging Kit takes advantage of this reaction to label virtually any recombinant antibody or protein containing the sortase recognition sequence. Labeling efficiency of greater than 90% is achieved by taking advantage of the highly active Sortase 5? Pentamutant. Simply combine your protein of interest with Sortase 5? and a polyglycine label|and incubate for 1 hour at 37°C. Purification columns are included to remove Sortase 5? and excess label. The kit also includes Ubiquitin-LPETGH6 and GGG-Clover as positive controls.
SKU | AMS.79392 |
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Size | 3 units of Sortase |
Application | Great for the site-specific labeling of recombinant antibodies and proteins. |
Gene synonyms | SrtA, sortase A, sortagging, S aureus |
Storage | Stable for at least 6 months from date of receipt when stored as directed. |
Shipping Temp | -80C (dry ice) |
Supplier Name | BPS |