Immunofluorescent staining of HT29 cells using anti-SIGLEC9 mouse monoclonal antibody (TA500382).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIGLEC9 (RC206674, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIGLEC9. Positive lysates LY402335 (100ug) and LC402335 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SIGLEC9 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-SIGLEC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500382)