HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNX9 (RC202822, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNX9. Positive lysates LY402520 (100ug) and LC402520 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SNX9 monoclonal antibody.
Anti-SNX9 mouse monoclonal antibody (TA501356) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SNX9 (RC202822 ).
HEK293T cells transfected with either RC202822 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SNX9 antibody (TA501356), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-SNX9 antibody (TA501356), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).