Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and SETD7-Knockout HeLa cells (KO, Cat# LC833317) were separated by SDS-PAGE and immunoblotted with anti-SETD7 monoclonal antibody TA503354 (1:200`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SETD7 (RC219244, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SETD7. Positive lysates LY403067 (100ug) and LC403067 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC219244 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SETD7 antibody (TA503354), and then analyzed by flow cytometry.