HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SETD7 (RC219244, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SETD7. Positive lysates LY403067 (100ug) and LC403067 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SETD7 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and SETD7-Knockout HeLa cells (KO, Cat# LC833317) were separated by SDS-PAGE and immunoblotted with anti-SETD7 monoclonal antibody TA503377 (1:500`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
HEK293T cells transfected with either RC219244 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SETD7 antibody (TA503377), and then analyzed by flow cytometry.