HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINE2 (RC204904, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINE2. Positive lysates LY416795 (100ug) and LC416795 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-SERPINE2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-SERPINE2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon).
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SERPINE2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA504718)
HEK293T cells transfected with either RC204904 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SERPINE2 antibody (TA504718), and then analyzed by flow cytometry.