Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-SRR monoclonal antibody (1:200).
Anti-SRR mouse monoclonal antibody (TA500974) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SRR (RC210359).
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-SRR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500974, Dilution 1:50)
Immunofluorescent staining of HepG2 cells using anti-SRR mouse monoclonal antibody (TA500974).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SRR (RC210359, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SRR. Positive lysates LY402897 (100ug) and LC402897 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either pCMV6-ENTRY SRR (RC210359) (Red) or empty vector control plasmid (Blue) were immunostained with anti-SRR mouse monoclonal (TA500974), and then analyzed by flow cytometry.