Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SCYL3 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-SCYL3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500493, Dilution 1:50)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCYL3 (RC204859, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCYL3. Positive lysates LY412474 (100ug) and LC412474 (20ug) can be purchased separately from OriGene.
Anti-SCYL3 mouse monoclonal antibody (TA500493) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SCYL3 (RC204859).